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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 6, 2005, pp. 631-637
Bioline Code: oc05137
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 6, 2005, pp. 631-637

 en Variability and genetic differentiation among Anopheles (Ano.) intermedius check for this species in other resources Chagas, 1908 and Anopheles (Ano.) mattogrossensis check for this species in other resources Lutz & Neiva, 1911 (Diptera: Culicidae) from the Brazilian Amazon
Joselita Maria Mendes dos Santos; Gloria Alicia Diaz Rodriguez; Juracy de Freitas Maia & Wanderli Pedro Tadei

Abstract

Anopheles intermedius check for this species in other resources and Anopheles mattogrossensis check for this species in other resources are Brazilian anopheline species belonging to the scarcely studied Anopheles subgenus. Few studies have been done on the genetic differentiation of these species. Both species have been found infected by Plasmodium and are sympatric with other anopheline species from the Nyssorhynchus subgenus. Eighteen enzymatic loci were analyzed in larval specimens of An. intermedius and An. mattogrossensis aiming to estimate the variability and genetic differentiation between these species. An. mattogrossensis population showed higher genetic variability (P = 44.4 and Ho = 0.081 ± 0.031) than that of An. intermedius (P = 33.3 and Ho = 0.048 ± 0.021). Most analyzed loci showed genotypic frequencies according to Hardy-Weinberg equilibrium, except for LAP1 and LAP2 in An. intermedius, and EST1 and PGM loci in An. mattogrossensis. The genetic distance between these species (D = 0.683) was consistent with the inter-specific values reported for Anopheles subgenus. We verified that the polymorphism and heterozygosity percentile values found in both species and compared to those in the literature, showed no relation between the level of isozyme variability and geographical distribution. The low variability found in these two species is probably more related to the niche they occupy than to their geographic distribution.

Keywords
Anopheles intermedius - Anopheles mattogrossensis - isozymes - malaria - Amazon

 
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