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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 7, 2005, pp. 729-731
Bioline Code: oc05153
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 7, 2005, pp. 729-731

 en SHORT COMMUNICATION- Predominance of Trypanosoma rangeli check for this species in other resources infection in children from a Chagas disease endemic area in the west-shore of the Panama canal
Azael Saldaña; Franklyn Samudio; Aracelis Miranda; Lissette M Herrera; Sara P Saavedra; Lorenzo Cáceres; Vicente Bayard & José E Calzada

Abstract

A total of 206 serum samples from children (3-14 years old) living in the Amador County (La Chorrera District, Province of Panama) were screened by indirect immunofluorescence antibody test (IFAT) for the presence of antibodies against Trypanosoma cruzi check for this species in other resources . Positive sera were confirmed by recombinant enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. The presence of blood trypanosomes was investigated by hemoculture and subsequently identify by a duplex polymerase chain reaction (PCR) followed by dot blot hybridization. The results indicated a prevalence of 9.7% for trypanosome infections, a seroprevalence of 2.9% against T. cruzi and a predominance of T. rangeli infection (6.8%). The immunological and clinical implications of these findings are discussed.

Keywords
Trypanosoma rangeli - children infection - Panama

 
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