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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 7, 2005, pp. 739-741
Bioline Code: oc05156
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 7, 2005, pp. 739-741

 en SHORT COMMUNICATION- Ocurrence of co-infection by Leishmania (Leishmania) chagasi check for this species in other resources and Trypanosoma (Trypanozoon) evansi check for this species in other resources in a dog in the state of Mato Grosso do Sul, Brazil
Elisa San Martin Mouriz Savani; Vania Lúcia Brandão Nunes; Eunice Aparecida Bianchi Galati; Tiago Moreno Castilho; Fernando Shiroma de Araujo; Iêda Maria Novaes Ilha; Maria Cecília Gibrail de Oliveira Camargo; Sandra Regina Nicoletti D'Auria & Lucile Maria Floeter-Winter

Abstract

A natural case of co-infection by Leishmania and Trypanosoma is reported in a dog ( Canis familiaris check for this species in other resources ) in south- western state of Mato Grosso do Sul, Brazil. Both amastigote and trypomastigote forms were observed after Giemsa staining of cytological preparations of the dog's bone marrow aspirate. No parasite was detected using medium culture inoculation of the sample. DNA obtained from the bone marrow aspirate sample and from the blood buffy coat was submitted to polymerase chain reaction (PCR) with a set of rDNA-based primers S4/S12. The nucleotide sequence of the PCR product was identical to that of Trypanosoma (Trypanozoon) evansi check for this species in other resources . The S4/S12 PCR was then used as template in a nested-PCR using a specific Leishmania set S17/S18 as primers, to explain the amastigote forms. The nucleotide sequence of the new PCR product was identical to that of Leishmania (Leishmania) chagasi check for this species in other resources . This case, as far as we know, is the first report of a dog co-infected with these parasites, suggesting that besides L. (L.) chagasi, the natural transmission of T. (T.) evansi occurs in the area under study.

Keywords
trypanosomatid identification - rDNA-based polymerase chain reaction - epidemiology - natural reservoirs - Brazil

 
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