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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 100, No. 7, 2005, pp. 765-769
Bioline Code: oc05161
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 100, No. 7, 2005, pp. 765-769

 en Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale check for this species in other resources
Flábio R Araújo; Valeska SP Melo; Carlos AN Ramos; Claudio R Madruga; Cleber O Soares; Raul H Kessler; Nalvo F Almeida; Graziela S Araújo; Leucio C Alves; Roberto AA Torres Júnior; Stênio P Fragoso; Paulo RC Arauco; Gisele Bacanelli; Maristeli B Oliveira & Lenita R Santos

Abstract

Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale check for this species in other resources were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99% for both tests) and specificities (100% for both tests) were confirmed with sera from cattle positive or negative for A. marginale antibodies, respectively, by immunofluorescent antibody test. By the analysis of 583 sera from cattle of three regions of the state of Pernambuco, Brazil, the agreement between both tests was high, with a kappa index of 0.89. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.

Keywords
Anaplasma marginale - indirect enzyme-linked immunosorbent assays - major surface proteins - Brazil

 
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