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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 1, 2006, pp. 39-45
Bioline Code: oc06008
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 1, 2006, pp. 39-45

 en Genetic structure of Triatoma venosa check for this species in other resources (Hemiptera: Reduviidae): molecular and morphometric evidence
Erika Vargas; Claudia Espitia; Carlos Patiño; Nestor Pinto; Germán Aguilera; Carlos Jaramillo; María Dolores Bargues & Felipe Guhl

Abstract

Triatoma venosa check for this species in other resources presents a restricted geographical distribution in America and is considered as a secondary vector of Chagas disease in Colombia and Ecuador. A total of 120 adult insects were collected in domestic and peridomestic habitats in an endemic area of the department of Boyacá, Colombia, in order to determine their genetic structure through morphometric and molecular techniques. The head and wings of each specimen were used for the analyses of size, shape, and sexual dimorphism. A significant sexual dimorphism was found, although no differences in size among the studied groups were detected. Differences were found in the analyzed structures except for male heads.

DNA was extracted from the legs in order to carry out the internal transcriber space-2 (ITS-2) amplification and the randon amplified polymorphic DNA (RAPD) analyses. Length polymorphisms were not detected in the ITS-2. Fst and Nm values were estimated (0.047 and 3.4, respectively). The high genetic flow found among the insects captured in the domicile and peridomiciliary environment does not permit a genetic differentiation, thus establishing the peridomicile as an important place for epidemiological surveillance.

Keywords
Triatoma venosa - geometric morphometry - internal transcriber space-2 - random amplified polymorphic DNA - vector control

 
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