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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 2, 2006, pp. 219-221
Bioline Code: oc06038
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 2, 2006, pp. 219-221

 en Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis check for this species in other resources DNA on serum samples from patients with paracoccidioidomycosis
Cecília Eugenia Charbel; José Eduardo Levi & José Eduardo Costa Martins

Abstract

The aim of this study was to demonstrate the DNA of Paracoccidioides brasiliensis check for this species in other resources in human serum samples of patients with paracoccidioidomycosis using the polymerase chain reaction (PCR). The diagnosis of paracoccidioidomycosis (PCM) was defined by microscopic observation of the fungus on direct exam or histopathology, culture, and serological positivity. DNA from serum of 33 patients with PCM was extracted and submitted to nested-PCR using primers from the gp 43 gene. Only one sample was positive on nested-PCR. We conclude that the prevalence of fungemia in patients with different clinical forms of PCM is low, limiting the use of serum DNA detection as an alternative diagnostic tool.

Keywords
paracoccidioidomycosis - Paracoccidioides brasiliensis - molecular diagnosis - serum - polymerase chain reaction

 
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