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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 4, 2006, pp. 427-430
Bioline Code: oc06076
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 4, 2006, pp. 427-430

 en Polymerase chain reaction with lesion scrapping for the diagnosis of human American tegumentary leishmaniasis
Eneide Aparecida Sabaini Venazzi; Andréa Claudia Bekner Silva Roberto; Ione Parra Barbosa-Tessmann; Paulo Donizeti Zanzarini; Maria Valdrinez Campana Lonardoni & Thaís Gomes Verzignassi Silveira

Abstract

The objective of this work was to compare the polymerase chain reaction (PCR) using lesion scrapping with other conventional techniques for the diagnosis of the American tegumentary leishmaniasis (ATL). For this, patients with cutaneous lesions suspected to be ATL were studied. The DNA was amplified with the MP1L/MP3H primers. From the 156 studied patients, 79 (50.6%) presented positive parasite direct search (PD), 81 (51.9%) had positive Montenegro skin test (MST), and 90 (57.7%) presented PD and/or MST positive. The PCR was positive in all of the positive-PD patients (100% sensitivity), in 91.1% of the positive PD and/or MST patients, and in 27.3% of the patients that presented negative PD and positive MST. The PCR positivity was similar to the PD (P = 0.2482) and inferior to the MST (P = 0.0455), and to the PD/MST association (P = 0.0133). The high PCR sensitivity, and positivity in those cases where the PD was negative, highlights the importance of this technique as an auxiliary tool for the diagnosis of ATL.

Keywords
American tegumentary leishmaniasis - laboratorial diagnosis - polymerase chain reaction

 
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