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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 4, 2006, pp. 451-453
Bioline Code: oc06079
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 4, 2006, pp. 451-453

 en Isolation of respiratory syncytial virus from nasopharyngeal aspirates stored at 20°C from one to fifteen months after collection
IFS Nunes & FEA Moura

Abstract

Cell culture isolation is used for recovering respiratory syncytial virus (RSV) from respiratory specimens. As RSV is a thermolabile virus, specimens destined for inoculation into cell culture require special transport, handling, and storage. The isolation rate of RSV from nasopharyngeal aspirates (NPA) stored at -20°C for one to 15 months after collection was investigated. A total of 126 samples considered positive for RSV by indirect fluorescence-antibody were tested by virus isolation in HEp-2 cell culture. RSV was isolated from 47/126 specimens (37.3%). These results show that RSV may be recovered from NPA stored at -20°C by cell culture.

Keywords
respiratory syncytial virus - HEp-2 cell culture - nasopharyngeal aspirates

 
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