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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 5, 2006, pp. 499-501
Bioline Code: oc06084
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 5, 2006, pp. 499-501

 en Application of biochemical and polymerase chain reaction assays for identification of Campylobacter check for this species in other resources isolates from non-human primates
Mônica de Castro Britto Vilardo; Jacqueline Darc da Silva Thomé; Wagner Thadeu Cardoso Esteves; Ana Luzia Lauria Filgueiras & Selma Soares de Oliveira

Abstract

A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni check for this species in other resources and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.

Keywords
Campylobacter - identification - non-human primates

 
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