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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 5, 2006, pp. 503-510
Bioline Code: oc06085
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 5, 2006, pp. 503-510

 en Latex constituents from Calotropis procera check for this species in other resources (R. Br.) display toxicity upon egg hatching and larvae of Aedes aegypti check for this species in other resources (Linn.)
Márcio Viana Ramos; Glaís de Paiva Bandeira; Cléverson Diniz Teixeira de Freitas; Nádia Accioly Pinto Nogueira; Nylane Maria Nunes Alencar; Petrônio Augusto Simão de Sousa & Ana Fontenele Urano Carvalho

Abstract

Calotropis procera check for this species in other resources R. Br. (Asclepiadaceae) is a well-known medicinal plant with leaves, roots, and bark being exploited by popular medicine to fight many human and animal diseases. This work deals with the fractionation of the crude latex produced by the green parts of the plant and aims to evaluate its toxic effects upon egg hatching and larval development of Aedes aegypti check for this species in other resources . The whole latex was shown to cause 100% mortality of 3rd instars within 5 min. It was fractionated into water-soluble dialyzable (DF) and non-dialyzable (NDF) rubber-free materials. Both fractions were partially effective to prevent egg hatching and most of individuals growing under experimental conditions died before reaching 2nd instars or stayed in 1st instars. Besides, the fractions were very toxic to 3rd instars causing 100% mortality within 24 h. When both fractions were submitted to heat-treatment the toxic effects were diminished considerably suggesting low thermostability of the toxic compounds. Polyacrylamide gel electrophoresis of both fractions and their newly fractionated peaks obtained through ion exchange chromatography or desalting attested the presence of proteins in both materials. When submitted to protease digestion prior to larvicidal assays NDF lost most of its toxicity but DF was still strongly active. It may be possible that the highly toxic effects of the whole latex from C. procera upon egg hatching and larvae development should be at least in part due to its protein content found in NDF. However the toxicity seems also to involve non protein molecules present in DF.

Keywords
dengue - larvicidal - laticiferous plants - plant proteins - tropical diseases

 
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