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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 5, 2006, pp. 565-571
Bioline Code: oc06096
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 5, 2006, pp. 565-571

 en Identification of the Boudicca and Sinbad retrotransposons in the genome of the human blood fluke Schistosoma haematobium check for this species in other resources
Claudia S Copeland; Fred A Lewis & Paul J Brindley

Abstract

Schistosomes have a comparatively large genome, estimated for Schistosoma mansoni check for this species in other resources to be about 270 megabase pairs (haploid genome). Recent findings have shown that mobile genetic elements constitute significant proportions of the genomes of S. mansoni and S. japonicum. Much less information is available on the genome of the third major human schistosome, S. haematobium. In order to investigate the possible evolutionary origins of the S. mansoni long terminal repeat retrotransposons Boudicca and Sinbad, several genomes were searched by Southern blot for the presence of these retrotransposons. These included three species of schistosomes, S. mansoni, S. japonicum, and S. haematobium, and three related platyhelminth genomes, the liver flukes Fasciola hepatica check for this species in other resources and Fascioloides magna check for this species in other resources and the planarian, Dugesia dorotocephala check for this species in other resources . In addition, Homo sapiens check for this species in other resources and three snail host genomes, Biomphalaria glabrata check for this species in other resources , Oncomelania hupensis check for this species in other resources , and Bulinus truncatus check for this species in other resources , were examined for possible indications of a horizontal origin for these retrotransposons. Southern hybridization analysis indicated that both Boudicca and Sinbad were present in the genome of S. haematobium. Furthermore, low stringency Southern hybridization analyses suggested that a Boudicca-like retrotransposon was present in the genome of B. truncatus, the snail host of S. haematobium.

Keywords
schistosome - long terminal report retrotransposon - mobile genetic element - reverse transcriptase - Bulinus

 
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