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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 6, 2006, pp. 625-633
Bioline Code: oc06106
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 6, 2006, pp. 625-633

 en Population genetic structure of the dengue mosquito Aedes aegypti check for this species in other resources in Venezuela
Flor Herrera; Ludmel Urdaneta; José Rivero; Normig Zoghbi; Johanny Ruiz; Gabriela Carrasquel; José Antonio Martínez; Martha Pernalete; Patricia Villegas; Ana Montoya; Yasmin Rubio-Palis & Elina Rojas

Abstract

The mosquito Aedes aegypti check for this species in other resources is the main vector of dengue in Venezuela. The genetic structure of this vector was investigated in 24 samples collected from eight geographic regions separated by up to 1160 km. We examined the distribution of a 359-basepair region of the NADH dehydrogenase subunit 4 mitochondrial gene among 1144 Ae. aegypti from eight collections. This gene was amplified by the polymerase chain reaction and tested for variation using single strand conformation polymorphism analysis. Seven haplotypes were detected throughout Venezuela and these were sorted into two clades. Significant differentiation was detected among collections and these were genetically isolated by distance.

Keywords
Aedes aegypti - genetic structure - dengue - single strand conformation polymorphism analysis - ND4 haplotypes

 
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