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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 6, 2006, pp. 693-696
Bioline Code: oc06118
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 6, 2006, pp. 693-696

 en Giardia duodenalis check for this species in other resources : analysis of secreted proteases upon trophozoite-epithelial cell interaction in vitro
Guadalupe Beatriz Rodríguez-Fuentes; Roberto Cedillo-Rivera; Rocío Fonseca-Liñán; Raúl Argüello-García; Onofre Muñoz; Guadalupe Ortega-Pierres & Lilián Yépez-Mulia

Abstract

Protease secretion by Giardia duodenalis check for this species in other resources trophozoites upon interaction with epithelial cells and its association with the parasite adhesion was studied in co-cultures of parasites with IEC6 epithelial cell monolayers in the presence or absence of protease inhibitors. Proteolytic activity in supernatants from trophozoites was enhanced when they were co-cultured with IEC6 cells. This activity was strongly inhibited by pre-incubation of live trophozoites with E-64 and TPCK and a concomitant inhibition of parasite adhesion to IEC6 cells was observed. These data suggest that trophozoites secrete cysteine-type proteases that play a role in the adhesion of G. duodenalis to epithelial cells.

Keywords
Giardia duodenalis - protease secretion - adhesion

 
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