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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 6, 2006, pp. 693-696
Bioline Code: oc06118
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 6, 2006, pp. 693-696

 en Giardia duodenalis check for this species in other resources : analysis of secreted proteases upon trophozoite-epithelial cell interaction in vitro
Guadalupe Beatriz Rodríguez-Fuentes; Roberto Cedillo-Rivera; Rocío Fonseca-Liñán; Raúl Argüello-García; Onofre Muñoz; Guadalupe Ortega-Pierres & Lilián Yépez-Mulia


Protease secretion by Giardia duodenalis check for this species in other resources trophozoites upon interaction with epithelial cells and its association with the parasite adhesion was studied in co-cultures of parasites with IEC6 epithelial cell monolayers in the presence or absence of protease inhibitors. Proteolytic activity in supernatants from trophozoites was enhanced when they were co-cultured with IEC6 cells. This activity was strongly inhibited by pre-incubation of live trophozoites with E-64 and TPCK and a concomitant inhibition of parasite adhesion to IEC6 cells was observed. These data suggest that trophozoites secrete cysteine-type proteases that play a role in the adhesion of G. duodenalis to epithelial cells.

Giardia duodenalis - protease secretion - adhesion

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