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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 7, 2006, pp. 721-724
Bioline Code: oc06121
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 7, 2006, pp. 721-724

 en Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil
Ana Maria Tavares Borges; José; Marcus Sócrates Teixeira; Paulo Sérgio Sucasas da Costa; Loreny Gimenes Giugliano; Fabíola Souza Fiaccadori; Rachel de Carvalho e Franco; Wilia Marta Elsner Diederichsen de Brito; José Paulo Gagliardi Leite & Divina das Dores de Paula Cardoso


The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period.

calicivirus - acute gastroenteritis - reverse transcriptase-polymerase chain reaction - Brazil

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