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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 7, 2006, pp. 801-807
Bioline Code: oc06134
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 7, 2006, pp. 801-807

 en The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax check for this species in other resources antibodies and its use in epidemiological surveys
Claudio R Madruga; Flábio R Araújo; Gustavo Cavalcante-Goes; Charles Martins; Ingá B Pfeifer; Laura R Ribeiro; Raul H Kessler; Cleber O Soares; Midori Miguita; Elaine PS Melo; Robson FC Almeida & Manoel MSC Lima Jr.

Abstract

There are data indicating that the distribution of Trypanosoma vivax check for this species in other resources in the Brazilian territory is expanding with potential to reach other areas, where the vectors are present. The detection of anti-trypanosomal antibodies in serum provides important information of the trypanosomal status in cattle herds. For this reason, an enzyme-linked immunosorbent assay (Tv-ELISA-Ab) with crude antigen from one Brazilian isolate of T. vivax was developed and evaluated. The sensitivity and specificity were respectively 97.6 and 96.9%. In the evaluation of cross-reactions, three calves inoculated with T. evansi trypimastigotes blood forms showed optical densities (OD) under the cut-off during the whole experimental period, except one at 45 days post-inoculation. With relation to Babesia bovis, B. bigemina, and Anaplasma marginale, which are endemic hemoparasites in the studied area, the cross-reactions were shown to be 5.7, 5.3, and 1.1%, respectively. The first serological survey of Pantanal and state of Pará showed that T. vivax is widespread, although regions within both areas had significantly different prevalences. Therefore, this Tv-ELISA-Ab may be a more appropriate test for epidemiological studies in developing countries because the diagnostic laboratories in most countries may be able to perform an ELISA, which is not true for polymerase chain reaction.

Keywords
Trypanosoma vivax - enzyme-linked immunosorbent assay - epidemiological survey - cattle - Pantanal - Brazil

 
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