search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. 8, 2006, pp. 851-856
Bioline Code: oc06140
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. 8, 2006, pp. 851-856

 en Single and concomitant experimental infections by Endotrypanum spp. check for this species in other resources and Leishmania (Viannia) guyanensis check for this species in other resources (Kinetoplastida: Trypanosomatidae) in the Neotropical sand fly Lutzomyia longipalpis check for this species in other resources (Diptera: Psychodidae)
André F Barbosa; Sandra MP Oliveira; Àlvaro L Bertho; Antonia MR Franco & Elizabeth F Rangel

Abstract

Lutzomyia longipalpis check for this species in other resources females received single and mixed infections with Endotrypanum and Leishmania. Two biological parameters were analyzed: the percentage of infected females and the distribution of flagellates in the gut of the females. The principal comparisons were performed between (1) two strains of Endotrypanum, (2) cloned versus primary sample of one strain of Endotrypanum, (3) Endotrypanum versus Leishmania guyanensis check for this species in other resources , and (4) the pattern of flagellates behaviour by optical microscopy in females with single or mixed infection versus the identification of parasites isolated from digestive tracts by isoenzyme electrophoresis. Flagellates of Endotrypanum showed distinct patterns of infection suggesting that there is variation between and within strains. The distribution of Endotrypanum and L. guyanensis differed significantly in relation to the colonization of the stomodeal valve. In co-infection with L. guyanensis, a large number of flagellates were seen to be plentifully infecting the stomodeal valve in significantly more specimens than in females infected by Endotrypanum only. However, the electrophoretic profiles of isoenzymes of parasites recovered from all co-infected specimens corresponded to Endotrypanum. This suggests that the mere correlation sand fly infection-biochemical analysis of isolates may induce parasitological incorrect consideration.

Keywords
Endotrypanum - Leishmania (Viannia) spp. - Lutzomyia longipalpis - flow cytometric cloning - co-infection

 
© Copyright 2006 - Instituto Oswaldo Cruz - Fiocruz
Alternative site location: http://memorias.ioc.fiocruz.br

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil