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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. s1, 2006, pp. 167-177
Bioline Code: oc06185
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. s1, 2006, pp. 167-177

 en A bacterial artificial chromosome library for Biomphalaria glabrata check for this species in other resources , intermediate snail host of Schistosoma mansoni check for this species in other resources
Coen M Adema; Mei-Zhong Luo; Ben Hanelt; Lynn A Hertel; Jennifer J Marshall; Si-Ming Zhang; Randall J DeJong; Hye-Ran Kim; David Kudrna; Rod A Wing; Cari Soderlund; Matty Knight; Fred A Lewis; Roberta Lima Caldeira; Liana K Jannotti-Passos; Omar dos Santos Carvalho & Eric S Loker

Abstract

To provide a novel resource for analysis of the genome of Biomphalaria glabrata check for this species in other resources , members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research Institute (NHGRI), produced a high quality bacterial artificial chromosome (BAC) library. The BB02 strain B. glabrata , a field isolate (Belo Horizonte, Minas Gerais, Brasil) that is susceptible to several strains of Schistosoma mansoni , was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library (BG_BBa) consists of 61824 clones (136.3 kb average insert size) and provides 9.05 ´ coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clones indicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads, 299860 nt) indicated that the genome of B. glabrata contains ~ 63% AT, and disclosed several novel genes, transposable elements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI at cost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.

Keywords
genomics - gene discovery - fingerprinting - schistosomiasis - medical malacology

 
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