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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. s1, 2006, pp. 323-326
Bioline Code: oc06209
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. s1, 2006, pp. 323-326

 en Functional complementation of a yeast knockout strain by Schistosoma mansoni check for this species in other resources Rho1 GTPase in the presence of caffeine, an agent that affects mutants defective in the protein kinase C signal transduction pathway
Pedro HN de Aguiar; Débora N Santos; Francisco P Lobo; Túlio M Santos; Andréa M Macedo; Sérgio DJ Pena; Carlos R Machado & Glória R Franco

Abstract

In a previous study, the Schistosoma mansoni check for this species in other resources Rho1 protein was able to complement Rho1 null mutant Saccharomyces cerevisiae check for this species in other resources cells at restrictive temperatures and under osmotic stress (low calcium concentration) better than the human homologue (RhoA). It is known that under osmotic stress, the S. cerevisiae Rho1 triggers two distinct pathways: activation of the membrane 1,3-b-glucan synthase enzymatic complex and activation of the protein kinase C1 signal transduction pathway, promoting the transcription of response genes. In the present work the SmRho1 protein and its mutants smrhoE97P, smrho1L101T, and smrhoE97P, L101T were used to try to clarify the basis for the differential complementation of Rho1 knockout yeast strain by the human and S. mansoni genes. Experiments of functional complementation in the presence of caffeine and in the presence of the osmotic regulator sorbitol were conducted. SmRho1 and its mutants showed a differential complementation of the yeast cells in the presence of caffeine, since smrhoE97P and smrhoE97P, L101T mutants showed a delay in the growth when compared to the yeast complemented with the wild type SmRho1. However, in the presence of sorbitol and caffeine the wild type SmRho1 and mutants showed a similar complementation phenotype, as they allowed yeast growth in all caffeine concentrations tested.

Keywords
Schistosoma mansoni - SmRho1 - protein kinase C1 - caffeine - Saccharomyces cerevisiae

 
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