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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 101, No. s1, 2006, pp. 355-357
Bioline Code: oc06216
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 101, No. s1, 2006, pp. 355-357

 en Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni
Edward José de Oliveira; Hermínia Yohko Kanamura; Kioko Takei; Rosario Dominguez Crespo Hirata; Nga Yen Nguyen & Mário Hiroyuki Hirata

Abstract

The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventional methodology. The signals increased considerably (p < 0.0003) on wells sensitized with P1 to P6 using alternative methodology. In contrast, the well coated with peptide P7 presented lower signal when compared with conventional methodology (p = 0.0019). These results, establish the basis for the application of synthetic peptides for laboratory diagnosis of schistosomiasis mansoni.

Keywords
Schistosoma mansoni - immunodiagnosis - synthetic peptides

 
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