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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 102, No. 1, 2007, pp. 41-47
Bioline Code: oc07006
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 102, No. 1, 2007, pp. 41-47

 en Evaluation of phenotypic and genotypic alterations induced by long periods of subculturing of Cryptococcus neoformans check for this species in other resources strains
Cavalcante, Sonia Cristina; Freitas, Roseli Santos; Vidal, Mônica Scarpelli Martinelli; Dantas, Kátia Cristina; Levi, José Eduardo & Martins, José Eduardo Costa

Abstract

Cryptococcus neoformans check for this species in other resources is an encapsulated fungal organism that can cause disease in apparently im-munocompetent, as well as immunocompromised, hosts. Since 1930, successive subculture has been used to preserve C. neoformans isolates in our Fungus Collection. In the 1970s, some of these Fungus Collection samples were selected to be subjected to a different methods of maintenance that of lyophilized. Our objective was to analyze C. neoformans isolates in order to make a comparative evaluation between these two methods of preservation. The overall aim of this study was to qualify the preservation technique used in our mycology laboratory since the technique used might affect the survival, stability and purity of the primary isolates in culture.

The samples were analyzed using classical mycology methods and using the randomly amplified polymorphic DNA technique.In the analysis of phenotypes and genotypes, the typical characteristics of C. neoformans were found to differ in relation to the different methods of preservation employed.

The aim of this study was to demonstrate the importance of selecting the appropriate method of preservation for fungus collections. This selection can affect the survival and purity of the cultures, and preserve the stability of their physiological, biochemical, and genetic characteristics.

Keywords
Cryptococcus neoformans - random amplified polymorphic DNA technique - freeze drying

 
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