The object of the present work was to identify the larval habitats of
Culex eduardoi
and to determine the microenvironmental conditions related to their presence in different artificial freshwater environments (temporary, semi-permanent, irrigation ditches, and drainage ditches) in tillable areas of Chubut Province, Argentina. This report represents the first record of
Cx. eduardoi from this Province and extends its range to latitude 45°S. Immature stages of
Cx. eduardoi were found in 8 out of 109 (7.3 %) freshwater habitats and were significantly more prevalent in semi-permanent water bodies. Positive sites had significantly larger surface areas and more vegetation cover than negative sites
