en |
Association of cytokine genetic polymorphism with hepatites B infection evolution in adult patients
Ribeiro, Cátia Silene Sversuti; Visentainer, Jeane Eliete Laguila & Moliterno, Ricardo Alberto
Abstract
The infection by the hepatitis B virus (HBV) has different forms of evolution, ranging from self-limited infection
to chronic hepatic disease. The objective of this study was to evaluate the influence of cytokine genetic
polymorphisms in the disease evolution. The patients were divided into two groups, one with chronic HBV (n =
30), and the other with self-limited infection (n = 41). The genotyping for TNF (–308), TGFB1 (+869, +915), IL-
10 (1082, –819, and –592), IL-6 (–174), and IFNG (+874) was accomplished by the PCR-SSP (polymerase chain
reaction with sequence specific primers technique using the One Lambda kit. Although no statistically significant
differences were found between the groups, the combination of TNF –308GG and IFNG +874TA was found
in a lower frequency in chronic patients than in individuals with self-limited infection (26.7 versus 46.3%; P =
0.079; OR = 0.40; IC95% = 0.14-1.11). In chronic patients with histological alterations it was not observed the
genotype TGFB1+869 C/C, against 24.4% in the self limited infection group (100 versus 75.6%; P = 0.096; OR
= 7.67; IC95% = 0.42-141.63). Further studies in other populations, and evaluation of a greater number of
individuals could contribute for a better understanding of the cytokine genetic polymorphism influence in HBV
infection evolution.
Keywords
cytokine gene polymorphism - hepatitis B - genetic association
|