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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 102, No. 4, 2007, pp. 455-462
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Bioline Code: oc07072
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 102, No. 4, 2007, pp. 455-462
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Typing of Mycobacterium tuberculosis strains isolated in Community Health Centers of Rio de Janeiro City, Brazil
Oelemann, Maraníbia C; Fontes, Amanda NB; Pereira, Marcia A da Silva; Bravin, Yolanda; Silva, Guida; Degrave, Wim; Carvalho, Anna Cristina C; Brito, Rossana C; Kritski, Afrânio L & Suffys, Philip N
Abstract
Fingerprinting of Mycobacterium tuberculosis strains from tuberculosis (TB) patients attended in Community
Health Centers (CHCs) of Rio de Janeiro was performed to verify possible risk factors for TB transmission.
A prospective community-based study was performed during the period of July 1996 to December 1996 by
collecting sputum samples of 489 patients in 11 different CHCs in four different planning areas (APs) of the city.
Bacteriological, clinical, and epidemiological information was collected and M. tuberculosis genotypes defined
after restriction fragment length polymorphism (IS6110-RFLP) and double repetitive element (DRE) fingerprinting
of RFLP-clustered cases. Risk factors for TB transmission were looked for using three levels of
cluster stringency. Among 349 (71%) positive cultures obtained, IS6110-RFLP typing could be performed on
strains from 153 different patients. When using identity of RFLP patterns as cluster definition, 49 (32%) of the
strains belonged to a cluster and none of the clinical or epidemiologic characteristics was associated with
higher clustering levels. However, higher clustering level was observed in the AP including the central region
of the city when compared to others. This strongly suggests that more recent transmission occurs in that area
and this may be related with higher incidence of TB and HIV in this region.
Keywords
tuberculosis - molecular epidemiology - IS6110-restriction fragment length polymorphism - double repetitive element
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