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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 102, No. 5, 2007, pp. 639-642
Bioline Code: oc07101
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 102, No. 5, 2007, pp. 639-642

 en Isolation and identification of bovine tuberculosis in a Brazilian herd (São Paulo)
Nassar, AFC; Miyashiro, S; Oliveira, CG; Pacheco, WA & Ogata, RA

Abstract

Mycobacterium was verified in animals from a Brazilian dairy herd, a total of 42 samples from 30 cows were submitted to culture and the isolated strains were analyzed by two polymerase chain reaction (PCR), the first specific for species belonging to the Mycobacterium complex (MTBC) and the other for differentiating M. tuberculosis from M. bovis. Twenty seven samples (64.3%) from 18 animals (60%) were positive for mycobacteria by culture, including samples from 15 retrofaryngeal lymphnodes (55.5%), 9 prescapular lymphnodes (33.3%), 2 lungs (7.4%), and 1 liver (3.7%). All isolated colonies were confirmed by PCR to contain MTBC organisms, and were identified as M. bovis by the same methodology.

Keywords
tuberculosis - bovine - Mycobacterium bovis - intradermal tuberculin test - isolation - polymerase chain reaction

 
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