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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 102, No. 5, 2007, pp. 655-658
Bioline Code: oc07105
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 102, No. 5, 2007, pp. 655-658

 en Isolation and molecular identification of Leishmania (Viannia) peruviana   check for this species in other resources from naturally infected Lutzomyia peruensis   check for this species in other resources (Diptera: Psychodidae) in the Peruvian Andes
Perez, J Enrique; Veland, Nicolas; Espinosa, Diego; Torres, Katherine; Ogusuku, Elena; Llanos-Cuentas, Alejandro; Gamboa, Dionicia & Arévalo, Jorge

Abstract

Leishmania (Viannia) peruviana was isolated from 1/75 Lutzomyia peruensis captured during May 2006 in an endemic cutaneous leishmaniasis region of the Peruvian Andes (Chaute, Huarochiri, Lima, Peru). Sand fly gut with promastigotes was inoculated into a hamster and the remaining body was fixed in ethanol. L. (Viannia) sp. was determined by polymerase chain reaction (PCR), and Leishmania species through molecular genotyping by PCR-restriction fragment length polymorphism analyses targeting the genes cpb and hsp70, resulting L. (V.) peruviana. The infected sand fly appeared 15 days after the rains finished, time expected and useful real time data for interventions when transmission is occurring.

Keywords
Leishmania peruviana - Lutzomyia peruensis - polymerase chain reaction-restriction fragment length polymorphism - Peru

 
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