The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania
amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis
ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis
ESTs was also determined in L. (L.) amazonensis
and L. (L.) major
. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.