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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 102, No. 6, 2007, pp. 707-711
Bioline Code: oc07113
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 102, No. 6, 2007, pp. 707-711

 en Analysis and chromosomal mapping of Leishmania ( Leishmania) amazonensis check for this species in other resources amastigote expressed sequence tags
Gentil, Luciana Girotto; Lasakosvitsch, Fernanda; da Silveira, José Franco; dos Santos, Márcia Regina Machado & Barbiéri, Clara Lúcia

Abstract

The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania ( Leishmania) amazonensis check for this species in other resources amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis ESTs was also determined in L. (L.) amazonensis and L. (L.) major. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.

Keywords
Leishmania (Leishmania) amazonensis - amastigotes - cDNA library

 
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