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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 102, No. 7, 2007, pp. 861-866
Bioline Code: oc07154
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 102, No. 7, 2007, pp. 861-866

 en The involvement of tetA and tetE tetracycline resistance genes in plasmid and chromosomal resistance of Aeromonasin Brazilian strains
Balassiano, Ilana Teruszkin; de Freire Bastos, Maria do Carmo; Madureira, Danielle Jannuzzi; da Silva, Iris Gripp; de Freitas-Almeida, Ângela Corrêa & de Oliveira, Selma Soares

Abstract

This study analyzed the involvement of tetA and tetE genes in the tetracycline resistance of 16 strains of genus Aeromonas, isolated from clinical and food sources. Polymerase chain reactions revealed that 37.5% of the samples were positive for tetA, and also 37.5% were tetE positive. One isolate was positive for both genes. Only the isolate A. caviae 5.2 had its resistance associated to the presence of a plasmid, pSS2. The molecular characterization of pSS2 involved the construction of its restriction map and the determination of its size. The digestion of pSS2 with HindIII originated two fragments (A and B) that were cloned separately into the pUC18 vector. The tetA gene was shown to be located on the HindIII-A fragment by PCR. After transforming a tetracycline-sensitive strain with pSS2, the transformants expressed the resistance phenotype and harbored a plasmid whose size was identical to that of pSS2. The results confirmed the association between pSS2 and the tetracycline resistance phenotype, and suggest a feasible dissemination of tetA and tetE among strains of Aeromonas. This study suggests the spreading tetA and tetE genes in Aeromonasin Brazil and describes a resistance plasmid that probably contributes to the dissemination of the resistance.

Keywords
Aeromona- plasmid - tetracycline - resistance determinants

 
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