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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 102, No. 7, 2007, pp. 871-876
Bioline Code: oc07156
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 102, No. 7, 2007, pp. 871-876

 en Transferrin uptake may occur through detergent-resistant membrane domains at the cytopharynx of Trypanosoma cruzi check for this species in other resources epimastigote forms
Corrêa, José R.; Atella, Georgia C.; Vargas, Camila & Soares, Maurilio J.

Abstract

Uptake of transferrin by epimastigote forms of the protozoan Trypanosoma cruzi check for this species in other resources occurs mainly through a cytostome/ cytopharynx, via uncoated endocytic vesicles that bud off from the bottom of the cytopharynx. We have here examined whether detergent-resistant membrane (DRM) domains might be involved in this process. Purified whole cell membrane fractions were assayed for cholesterol levels and used in dot blot analyses. Detergent-resistant membrane markers (cholera B toxin and anti-flotillin-1 antibody) presented positive reaction by dot blots in cholesterol-rich/ protein-poor membrane sub-fractions. The positive dot blot fraction was submitted to lipid composition analysis, showing composition similar to that of raft fractions described for other eukaryotic cells. Immunofluorescence assays allowed the localization of punctual positive signal for flotillin-1, matching the precise cytostome/ cytopharynx location. These data were confirmed by immunofluorescence assays with the co-localization of flotillin-1 and the transferrin uptake site. Our data suggest that DRM domains occur and are integrated at the cytostome/ cytopharynx of T. cruzi epimastigotes, being the main route for transferrin uptake.

Keywords
cytopharynx - detergent-resistant membrane - endocytosis - Trypanosoma cruzi - ultrastructure

 
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