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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 102, No. 8, 2007, pp. 913-918
Bioline Code: oc07166
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 102, No. 8, 2007, pp. 913-918

 en First isolation of microorganisms from the gut diverticulum of Aedes aegypti check for this species in other resources (Diptera: Culicidae): new perspectives for an insect-bacteria association
Gusmão, Desiely Silva; Santos, Adão Valmir; Marini, Danyelle Cristine; de Souza Russo, Érica; Peixoto, Anelise Maria Dias; Bacci Júnior, Maurício; Berbert-Molina, Marília Amorim & Lemos, Francisco José Alves

Abstract

We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti check for this species in other resources was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were: Bacillus sp., Bacillus subtilis check for this species in other resources and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica check for this species in other resources .

Keywords
Aedes aegypti - gut diverticulum - microbiota - Serratia sp.

 
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