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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 102, No. 8, 2007, pp. 999-1002
Bioline Code: oc07178
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 102, No. 8, 2007, pp. 999-1002

 en Short Communication - Evaluation of a modified culture medium for Borrelia burgdorferi check for this species in other resources sensu lato
Rodríguez, Islay; Lienhard, Reto; Gern, Lise; Veuve, Marie Colette; Jouda, Fatima; Siegrist, Hans H.; Fernández, Carmen & Rodríguez, José Enrique

Abstract

The aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the culture of Borrelia strains, comparing the growth of individual strains in this medium and in the BSK-H medium, and the protein profile and antigenic characteristics of Borrelia proteins expressed in these media. A qualitative evaluation of growth of Borrelia species was made with acceptable results (morphology and motility), but during a quantitative evaluation using the three main genospecies of Borrelia, the better results were obtained with a B. burgdorferi sensu stricto strain. The modified medium did not enable the growth of a B. afzelii strain. The protein profile and antigenic characteristic of the expressed proteins in the modified medium were studied with satisfactory results. These results suggest the modified medium as an alternative for the cultivation of Borrelia strains, with some limitations, in poorly-resourced laboratories.

Keywords
Borrelia - new medium - BSK-H medium - proteins

 
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