search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 1, 2008, pp. 60-65
Bioline Code: oc08009
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 1, 2008, pp. 60-65

 en More about the role of 2,6-dichlorophenol in tick courtship: identification and olfactometer bioassay in Amblyomma cajennense check for this species in other resources and Rhipicephalus sanguineus check for this species in other resources
Louly, Carla Cristina Braz; Silveira, Diana da Nóbrega; Soares, Sara Fernandes; Ferri, Pedro Henrique; de Melo, Ariane Carla Campos & Borges, Lígia Miranda Ferreira

Abstract

This study aimed to identify 2,6-dichlorophenol (2,6-DCP) in Amblyomma cajennense check for this species in other resources and to evaluate its role in A. cajennense and Rhipicephalus sanguineus check for this species in other resources courtship. Hexanic extract from attractive females was purified by solid phase extraction and the phenol was identified by the single ion monitoring method using GC/MS. In an olfactometer, the responses of A. cajennense and R. sanguineus males to females, control rubber septa or rubber septa impregnated with 2,6-DCP at 50, 500, and 5000 ng, respectively, were studied. 2,6-DCP was identified in A. cajennense extract and the males oriented themselves toward the concentration of 500 ng. These septa and the females were recognized as copula partners. The septa treated with 2,6-DCP did not attract and were not even recognized by the R. sanguineus males, whereas the females were recognized. Due to the presence of 2,6-DCP in A. cajennense and the results of biological bioassays, it was concluded that this compound acts as an attractant and mounting sex pheromone in this tick, but it does not play any role in R. sanguineus courtship.

Keywords
2,6-dichlorophenol - sex pheromone - Ixodidae - olfactometer bioassay - identification

 
© Copyright 2008 Instituto Oswaldo Cruz - Fiocruz
Alternative site location: http://memorias.ioc.fiocruz.br

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil