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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 1, 2008, pp. 106-108
Bioline Code: oc08017
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 1, 2008, pp. 106-108

 en Molecular paleoparasitological diagnosis of Ascaris check for this species in other resources sp. from coprolites: new scenery of ascariasis in pre-Columbian South America times
Leles, Daniela; Araújo, Adauto; Ferreira, Luiz Fernando; Vicente, Ana Carolina Paulo & Iñiguez, Alena Mayo


Paleoparasitological studies using microscopy showed that Ascaris check for this species in other resources and Trichuris trichiura check for this species in other resources are the human intestinal parasites most found in archaeological sites. However, in pre-Columbian South American archaeological sites, Ascaris is rare. In this work we standardized a molecular methodology for Ascaris diagnosis directly from ancient DNA retrieved from coprolites. Using cythochrome b gene (142 bp) target, ancient DNA sequences were retrieved from South American samples, negative by microscopy. Moreover, the methodology applied was sensitive enough to detect ancient DNA extracted from 30 Ascaris eggs from an European coprolite. These results revealed a new scenery for the paleodistribution of Ascaris in South America.

Ascaris - molecular paleoparasitological diagnosis - paleodistribution

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