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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 103, No. 1, 2008, pp. 112-114
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Bioline Code: oc08019
Full paper language: English
Document type: Short Communication
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 1, 2008, pp. 112-114
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A new miracidia hatching device for diagnosing schistosomiasis
Jurberg, Arnon D.; de Oliveira, Áureo A.; Lenzi, Henrique L. & Coelho, Paulo Marcos Z.
Abstract
It is still imperative to develop a parasitological technique highly sensitive for diagnosing schistosomiasis in epidemiological and individual surveys. A simple and cheap hatching device with a collecting container was manufactured and tested under experimental conditions. Twelve Kato-Katz slides were performed as golden standard for comparison. Quantitative results can be carried out by counting miracidia in a plate and parasite load can be calculated (miracidia/gram of feces). Statistically significant values were higher in the hatching test. More sensitive results, with statistical significance, were achieved using 1.5 g of feces (which corresponds to 36 Kato-Katz slides) than by using the Kato-Katz method. Advantages of this technique and its limitations are presented.
Keywords
Schistosoma mansoni - diagnosis - feces
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