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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 3, 2008, pp. 221-235
Bioline Code: oc08051
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 3, 2008, pp. 221-235

 en New finding of Giardia intestinalis check for this species in other resources (Eukaryote, Metamonad) in Old World archaeological site using immunofluorescence and enzyme-linked immunosorbent assays
Le Bailly, Matthieu; Gonçalves, Marcelo LC; Harter-Lailheugue, Stéphanie; Prodéo, Frédéric; Araujo, Adauto & Bouchet, Françoise

Abstract

In this study, nine organic sediment samples from a medieval archaeological site at Pineuilh, France, were examined for Giardia intestinalis check for this species in other resources using two commercially available immunological kits [enzyme-linked immuno sorbent and immunofluorescence (IFA) assays]. Both techniques detected G. intestinalis in one sample, dated to 1,000 Anno Domini. This is the first time IFA was successfully used to detect protozoa in Old World archaeological samples. Such immunological techniques offer important perspectives concerning ancient protozoa detection and identification.

Keywords
paleoparasitology - ELISA - IFA - Giardia intestinalis

 
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