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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 3, 2008, pp. 221-235
Bioline Code: oc08053
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 3, 2008, pp. 221-235

 en Genetic characterization of morphologically variant strains of Paracoccidioides brasiliensis check for this species in other resources
Borba, Cintia de Moraes; Correia, Janaina; Vinhas, Ester; Martins, Albert; Alves, Beatriz Costa Aguiar; Unkles, Sheila; Kinghorn, James Robertson & Lucena-Silva, Norma

Abstract

Molecular characterization of Paracoccidioides brasiliensis check for this species in other resources variant strains that had been preserved under mineral oil for decades was carried out by random amplified polymorphic DNA analysis (RAPD). On P. brasiliensis variants in the transitional phase and strains with typical morphology, RAPD produced reproducible polymorphic amplification products that differentiated them. A dendrogram based on the generated RAPD patterns placed the 14 P. brasiliensis strains into five groups with similarity coefficients of 72%. A high correlation between the genotypic and phenotypic characteristics of the strains was observed. A 750 bp-RAPD fragment found only in the wild-type phenotype strains was cloned and sequenced. Genetic similarity analysis using BLASTx suggested that this RAPD marker represents a putative domain of a hypothetical flavin-binding monooxygenase (FMO)-like protein of Neurospora crassa check for this species in other resources .

Keywords
FMO - Paracoccidioides brasiliensis - RAPD-PCR

 
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