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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 4, 2008, pp. 347-350
Bioline Code: oc08060
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 4, 2008, pp. 347-350

 en Transcription of meiotic-like-pathway genes in Giardia intestinalis check for this species in other resources
Melo, Sandra P; Gómez, Vanessa; Castellanos, Isabel C; Alvarado, Magda E; Hernández, Paula C; Gallego, Amanda & Wasserman, Moisés

Abstract

The reproductive mechanism of Giardia intestinalis check for this species in other resources , considered one of the earliest divergent eukaryotes, has not been fully defined yet. Some evidence supports the hypothesis that Giardia check for this species in other resources is an exclusively asexual organism with a clonal population structure. However, the high genetic variability, the variation in ploidy during its life cycle, the low heterozygosity and the existence of genes involved in the meiotic-like recombination pathway in the parasite's genome cast doubt on exclusively asexual nature of Giardia. In this work, semiquantitative RT-PCR analysis was used to assess the transcription pattern of three meiosis-like-specific genes involved in homologues recombination: dmc1, hop1 and spo11. The mRNAs were amplified during the parasite's differentiation processes, encystation and excystation, and expression was found at each stage of its life cycle. A semiquantitative assessment also suggests that expression of some of the genes is regulated during encystation process.

Keywords
Giardia intestinalis - dmc1 - hop1 - spo11 - meiosis

 
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