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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 4, 2008, pp. 412-414
Bioline Code: oc08072
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 4, 2008, pp. 412-414

 en Isolation and molecular identification of Leishmania chagasi check for this species in other resources from a bat (Carollia perspicillata) in northeastern Venezuela
De Lima, Hector; Rodríguez, Noris; Barrios, Miguel A; Ávila, Ángela; Cañizales, Israel & Gutiérrez, Saúl

Abstract

This report describes the isolation of a Leishmania chagasi check for this species in other resources strain from a bat (Carollia perspicillata), and its identification using biological methods and molecular characterization. The parasites were isolated in an artificial culture medium from a blood sample extracted from a bat heart. The isolate was then inoculated into the footpads of Balb/c mice, which subsequently developed a typical nodular leishmanial lesion; the parasites were confirmed as Leishmania check for this species in other resources by smear and histopathology. Molecular characterization of the parasites was performed by polymerase chain reaction with species-specific primers, kDNA restriction pattern following Hae III endonuclease digestion and dot blot hybridization using a kDNA probe. This report demonstrates that bats can be hosts for L. chagasi species and suggests the need for studies to determine whether they may be involved in foci of visceral leishmaniasis.

Keywords
Leishmania chagasi - bats - molecular biology - reservoir - Venezuela

 
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