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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 103, No. 5, 2008, pp. 501-503
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Bioline Code: oc08089
Full paper language: English
Document type: Short Communication
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 5, 2008, pp. 501-503
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Cheap, rapid and efficient DNA extraction method to perform multilocus microsatellite genotyping on all Schistosoma mansoni stages
Beltran, S; Galinier, R; Allienne, JF & Boissier, J
Abstract
Schistosomes are endoparasites causing a serious human disease called schistosomiasis. The quantification of parasite genetic diversity is an essential component to understand the schistosomiasis epidemiology and disease transmission patterns. In this paper, we propose a novel assay for a rapid, low costly and efficient DNA extraction method of egg, larval and adult stages of Schistosoma mansoni. One euro makes possible to perform 60,000 DNA extraction reactions at top speed (only 15 min of incubation and 5 handling steps).
Keywords
Schistosoma mansoni - DNA extraction - microsatellites
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