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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 5, 2008, pp. 504-506
Bioline Code: oc08090
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 5, 2008, pp. 504-506

 en Serine protease activities in Oxysarcodexia thornax check for this species in other resources (Walker) (Diptera: Sarcophagidae) first instar larva
Cuervo, Patrícia; Mesquita-Rodrigues, Camila; Levy, Claudia Masini d'Avila; Britto, Constança; Pires, Fabiano Araújo; Gredilha, Rodrigo; Alves, Carlos Roberto & Batista de Jesus, Jose

Abstract

We report for the first time the expression of multiple protease activities in the first instar larva (L1) of the flesh fly Oxysarcodexia thornax check for this species in other resources (Walker). Zymographic analysis of homogenates from freshly obtained L1 revealed a complex proteolytic profile ranging from 21.5 to 136 kDa. Although some activities were detected at pH 3.5 and 5.5, the optimum pH for most of the proteolytic activities was between pH 7.5 and 9.5. Seven of 10 proteases were completely inactivated by phenyl-methyl sulfonyl-fluoride, suggesting that main proteases expressed by L1 belong to serine proteases class. Complete inactivation of all enzymatic activities was obtained using N-p-Tosyl-L-phenylalanine chloromethyl ketone (100 µM), a specific inhibitor of chymotrypsin-like serine proteases.

Keywords
Oxysarcodexia thornax - Sarcophagidae - chymotrypsin-like serine proteases

 
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