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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 7, 2008, pp. 683-689
Bioline Code: oc08124
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 7, 2008, pp. 683-689

 en The redox potential interferes with the expression of laminin binding molecules in Bacteroides fragilis check for this species in other resources
Ferreira, Eliane de Oliveira; Yates, Edwin Alexander; Goldner, Morris; Vommaro, Rossiane Cláudia; Filho, Fernando Costa e Silva; Petrópolis, Débora Barreiros & Domingues, Regina MC Pilotto


The Bacteroides fragilis check for this species in other resources ATCC strain was grown in a synthetic media with contrasting redox potential (Eh) levels [reduced (-60 mV) or oxidised (+100mV)] and their adhesion capacity to extracellular matrix components was evaluated. The strain was capable of adhering to laminin, fibronectin, fibronectin + heparan sulphate and heparan sulphate. A stronger adherence to laminin after growing the strain under oxidising conditions was verified. Electron microscopy using ruthenium red showed a heterogeneous population under this condition. Dot-blotting analyses confirmed stronger laminin recognition by outer membrane proteins of cells cultured at a higher Eh. Using a laminin affinity column, several putative laminin binding proteins obtained from the cultures kept under oxidising (60 kDa, 36 kDa, 25 kDa and 15 kDa) and reducing (60 kDa) conditions could be detected. Our results show that the expression of B. fragilis surface components that recognise laminin are influenced by Eh variations.

Bacteroides fragilis - adhesion - redox potential - laminin binding proteins - outer membrane proteins

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