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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 7, 2008, pp. 731-733
Bioline Code: oc08131
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 7, 2008, pp. 731-733

 en Characterization of Shigella check for this species in other resources spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
Silva, Tatiane; Nogueira, Paulo Afonso; Magalhães, Gleiciene Félix; Grava, Andréa Fagundes; da Silva, Luiz Hildebrando Pereira & Orlandi, Patrícia Puccinelli

Abstract

The incidence of Shigella check for this species in other resources spp. was assessed in 877 infants from the public hospital in Rondônia (Western Amazon region, Brazil) where Shigella represents the fourth cause of diarrhea. Twenty-five isolates were identified: 18 were Shigella flexneri check for this species in other resources , three Shigella sonnei check for this species in other resources , three Shigella boydii check for this species in other resources and one Shigella dysenteriae check for this species in other resources . With the exception of S. dysenteriae, all Shigella spp. isolated from children with diarrhea acquired multiple antibiotic resistances. PCR detection of ipa virulence genes and invasion assays of bloody diarrhea and fever (colitis) were compared among 25 patients testing positive for Shigella. The ipaH and ipaBCD genes were detected in almost all isolates and, unsurprisingly, all Shigella isolates associated with colitis were able to invade HeLa cells. This work alerts for multiple antibiotic resistant Shigella in the region and characterizes presence of ipa virulence genes and invasion phenotypesin dysenteric shigellosis.

Keywords
Shigella spp. - Brazil - multiple antibiotic resistance - diarrhea - ipa genes

 
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