Nucleotide sequences of the internal transcribed spacer 2 (ITS2) rDNA and partial sequences of the cytochrome coxidase subunit I (COI) mtDNA and
white gene nDNA were obtained from specimens of
Anopheles nuneztovari
A collected in Macapá (state of Amapá), Óbidos, Prainha and Almeirim (state of Pará), Itacoatiara and Parintins (state of Amazonas), Brazil, and compared with previously published sequences of
A. nuneztovari s.l. Results of the Bayesian phylogenetic analyses performed using either COI or combined ITS2, COI and white gene sequences suggest that
An. nuneztovari B/C is distinct from specimens obtained in the Amazonas/Solimões River basin.
Anopheles goeldii
, currently in synonymy with
An. nuneztovari, was described from individuals collected in Belterra (= Fordlândia) in the Tapajós River, state of Pará, Southern Amazonas River. Morphological comparisons of the characteristics of the male genitalia indicated that
An. nuneztovari A and
An. goeldii are similar but distinct from
An. nuneztovariB/C by the apex of the aedeagus. In considering the results of the phylogenetic analyses and morphological comparisons,
An. goeldii is resurrected from synonymy with
An. nuneztovari. Additionally,
Anopheles dunhamiis
reported for the first time in Parintins. This species can be distinguished from
An. goeldiiby characters of the male genitalia and molecular data.