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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 8, 2008, pp. 813-818
Bioline Code: oc08146
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 8, 2008, pp. 813-818

 en Primary Endemic Cryptococcosis Gattii by Molecular Type VGII in the State of Pará, Brazil
dos Santos, Wallace Raimundo A.; Meyer, Wieland; Wanke, Bodo; Costa, Solange P.S. Evangelista; Trilles, Luciana; Nascimento, Jose Luiz M.; Medeiros, Rita; Morales, Bernardina Pernarrieta; Bezerra, Claudia de Carvalho Falci; de Macêdo, Regina Célia Lima; Ferreira, Silvana O.; Barbosa, Gláucia Gonçalves; Perez, Mauricio A.; Nishikawa, Marília Martins & Lazéra, Márcia dos Santos

Abstract

In order to study the infectious agents causing human disseminated cryptococcosis in the state of Pará, North Brazil, 56 isolates of Cryptococcus check for this species in other resources spp. (54 isolated from cerebral spinal fluid and two from blood cultures) from 43 cases diagnosed between 2003-2007 were analysed. The species were determined through morphological and physiological tests and genotypes were determined by URA5-RFLP and PCR-fingerprinting (wild-type phage M13). The following species and genotypes were identified: Cryptococcus neoformans check for this species in other resources VNI (28/56, 50%), Cryptococcus gattii check for this species in other resources VGII (25/56, 44.64%) and C. gattii VGI (3/56, 5.26%). The genotype VNI occurred in 12 out of 14 HIV-positive adults, whereas the genotype VGII occurred in 11 out of 21 HIV-negative adults (p < 0.02, OR = 6.6 IC95% 0.98-56.0). All patients less than 12 years old were HIV negative and six cases were caused by the VGII genotype, one by the VGI and one by VNI. Therefore, endemic primary mycosis in HIV-negative individuals, including an unexpectedly high number of children, caused by the VGII genotype deserves further study and suggests the need for surveillance on cryptococcal infection in the state of Pará, Eastern Amazon.

Keywords
Cryptococcus gattii - VGII genotype - endemic mycosis - children - state of Pará

 
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