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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 103, No. 8, 2008, pp. 836-838
Bioline Code: oc08150
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 103, No. 8, 2008, pp. 836-838

 en Multiplex-PCR Serotyping of Listeria Monocytogenes check for this species in other resources Isolated From Human Clinical Specimens
de Vasconcelos, Rafaela Moledo; de Almeida, Antonio Eugenio Castro Cardoso; Hofer, Ernesto; da Silva, Nicéa Magaly Matias & Marin, Victor Augustus

Abstract

The genus Listeria is composed of six species of which Listeria monocytogenes check for this species in other resources is considered the single pathogenic species that causes listeriosis in humans. Of the 13 serovars of L. monocytogenes, 1/2a, 1/2b and 4b are responsible for the majority of clinical cases. The aim of this work was to detect L. monocytogenes in the cerebrospinal fluid sample of premature newborns and to characterize this sample using biotyping, serotyping and molecular typing. The results indicated the presence of L. monocytogenes in the clinical sample studied. Moreover, the isolate was identified as the 4b serovar that was characterized by the presence of a unique 691 bp band after analysis using the Multiplex-PCR technique. The results of repeated Multiplex-PCR and sequencing have indicated that the L. monocytogenes isolate was an atypical 4b serovar, which is the first time this finding has been reported.

Keywords
Listeria monocytogenes - listeriosis - Multiplex-PCR - cerebrospinal fluid

 
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