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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. 1, 2009, pp. 118-120
Bioline Code: oc09018
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. 1, 2009, pp. 118-120

 en Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
Marinheiro, Juliana Cristina; Sanalios, Roberta Braga; dos Santos, Daniela Carvalho; da Costa, Cristovão Alves & Hársi, Charlotte Marianna

Abstract

Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.

Keywords
human adenovirus - human respiratory syncytial virus - acute respiratory infection - polymerase chain reaction

 
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