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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. 3, 2009, pp. 427-433
Bioline Code: oc09067
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. 3, 2009, pp. 427-433

 en Population structure and diversity of the pathogenic fungus Aspergillus fumigatus check for this species in other resources isolated from different sources and geographic origins
Duarte-Escalante, Esperanza; Delhaes, Laurence; Reyes-Montes, María del Rocío; Zúñiga, Gerardo; Ramírez, Oscar Nava; Córdoba, Susana; Refojo, Nicolás & Arenas, Roberto

Abstract

Fifty-five clinical and environmental Aspergillus fumigatus check for this species in other resources isolates from Mexico, Argentina, France and Peru were analyzed to determine their genetic variability, reproductive system and level of differentiation using amplified fragment length polymorphism markers. The level of genetic variability was assessed by measuring the percentage of polymorphic loci, number of effective alleles, expected heterozygocity and by performing an association index test (IA). The degree of genetic differentiation and variation was determined using analysis of molecular variance at three levels. Using the paired genetic distances, a dendrogram was built to detect the genetic relationship among alleles. Finally, a network of haplotypes was constructed to determine the geographic relationship among them. The results indicate that the clinical isolates have greater genetic variability than the environmental isolates. The IA of the clinical and environmental isolates suggests a recombining population structure. The genetic differentiation among isolates and the dendrogram suggest that the groups of isolates are different. The network of haplotypes demonstrates that the majority of the isolates are grouped according to geographic origin.

Keywords
Aspergillus fumigatus - AFLP - genetic diversity - polymorphisms

 
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