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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. 3, 2009, pp. 473-480
Bioline Code: oc09075
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. 3, 2009, pp. 473-480

 en Cl gene cluster encoding several small nucleolar RNAs: a comparison amongst trypanosomatids
Nocua, Paola; Gómez, Carolina; Cuervo, Claudia & Puerta, Concepción

Abstract

Small nucleolar RNAs (snoRNAs) are small non-coding RNAs that modify RNA molecules such as rRNA and snRNA by guiding 2'-O-ribose methylation (C/D box snoRNA family) and pseudouridylation reactions (H/ACA snoRNA family). H/ACA snoRNAs are also involved in trans-splicing in trypanosomatids. The aims of this work were to characterise the Cl gene cluster that encodes several snoRNAs in Trypanosoma rangeli check for this species in other resources and compare it with clusters from Trypanosoma cruzi check for this species in other resources , Trypanosoma brucei check for this species in other resources , Leishmania major check for this species in other resources , Leishmania infantum check for this species in other resources , Leishmania braziliensis check for this species in other resources and Leptomonas collosoma check for this species in other resources . The T. rangeli Cl gene cluster is an 801 base pair (bp) repeat sequence that encodes three C/D (Cl1, Cl2 and Cl4) and three H/ACA (Cl3, Cl5 and Cl6) snoRNAs. In contrast to T. brucei, the Cl3 and Cl5 homologues have not been annotated in the Leishmania or T. cruzi genome projects (http//:www.genedb.org). Of note, snoRNA transcribed regions have a high degree of sequence identity among all species and share gene synteny. Collectively, these findings suggest that the Cl cluster could constitute an interesting target for therapeutic (gene silencing) or diagnostic intervention strategies (PCR-derived tools).

Keywords
C/D snoRNA - H/ACA snoRNA - rRNA - Trypanosoma rangeli - trypanosomatid

 
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