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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. s1, 2009, pp. 270-274
Bioline Code: oc09117
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. s1, 2009, pp. 270-274

 en α-N-Acetylglucosamine-Linked O-Glycans of Sialoglycoproteins (Tc-Mucins) From Trypanosoma Cruzi check for this species in other resources Colombiana Strain
Todeschini, Adriane R.; de Almeida, Eliane G.; Agrellos, Orlando A.; Jones, Christopher; Previato, José O. & Mendonça-Previato, Lucia

Abstract

Trypanosoma cruzi check for this species in other resources sialoglycoproteins (Tc-mucins) are mucin-like molecules linked to a parasite membrane via a glycosylphosphatidylinositol anchor. We previously determined the structures of Tc-mucin O-glycan domains from several T. cruzi strains and observed significant differences among them. We now report the amino acid content and structure of Tc-mucin O-glycan chains from T. cruzi Colombiana, a strain resistant to common trypanocidal drugs. Amino acid analysis demonstrated the predominance of threonine residues (42%) and helped to identify the O-glycans as belonging to a Tc-mucin family that contain a β-galactofuranose (β-Galf) residue attached to an α-N-acetylglucosamine (α-GlcNAc) O-4, with the most complex glycan, a pentasaccharide-GlcNAc-ol with a branched trigalactopyranose chain, on the GlcNAc O-6. The presence of β-Galf on O-glycans from T. cruzi Colombiana mucins supports the use of glycosylation as a phylogenetic marker for the classification of Colombiana in the T. cruzi I group.

Keywords
Trypanosoma cruzi - NMR spectroscopy - glycoconjugate - Colombiana strain - mucins - sialic acid

 
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