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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. 4, 2009, pp. 644-648
Bioline Code: oc09144
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. 4, 2009, pp. 644-648

 en Resistance of melanized yeast cells of Paracoccidioides brasiliensis check for this species in other resources to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18
da Silva, Marcelo Barbosa; Thomaz, Luciana; Marques, Alexandre Ferreira; Svidzinski, Artur E; Nosanchuk, Josh D; Casadevall, Arturo; Travassos, Luiz R & Taborda, Carlos P

Abstract

Paracoccidioides brasiliensis check for this species in other resources , a thermal dimorphic fungal pathogen, produces a melanin-like pigment in vitro and in vivo. We investigated the involvement of carbohydrates and monoclonal antibody to CD18, on phagocytosis inhibition, involving macrophage receptors and the resistance of melanized fungal cells to chemically generated nitric oxide (NO), reactive oxygen species (ROS), hypochlorite and H2O2. Our results demonstrate that melanized yeast cells were more resistant than nonmelanized yeast cells to chemically generated NO, ROS, hypochlorite and H2O2, in vitro. Phagocytosis of melanized yeast cells was virtually abolished when mannan, N-acetyl glucosamine and anti-CD18 antibody were added together in this system. Intratracheal infection of BALB/c mice, with melanized yeast cells, resulted in higher lung colony forming units, when compared to nonmelanized yeast cells. Therefore, melanin is a virulence factor of P. brasiliensis.

Keywords
melanin - P. brasiliensis - antimicrobial oxidants - anti-CD18 antibody

 
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