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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 104, No. 4, 2009, pp. 655-658
Bioline Code: oc09146
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 104, No. 4, 2009, pp. 655-658

 en The circulation of human astrovirus genotypes in the Central West Region of Brazil
Silva, Paula Andreia; Santos, Rodrigo Alessandro Tôgo; Costa, Paulo Sérgio Sucasas; Teixeira, José Marcus Sócrates; Giugliano, Loreny Gimenes; Andreasi, Márcia Sueli Assis; Leite, José Paulo Gagliardi; Schreier, Ecakart & Cardoso, Divina das Dôres de Paula

Abstract

Out of 1,588 faecal samples of children taken from three locations of the Central West Region of Brazil, 57 were positive for astroviruses (HAstVs) using reverse transcription-polymerase chain reaction (RT-PCR). They were genotyped by nested RT-PCR and/or genomic sequencing. HAstV-1 (42.8%), HAstV-2 (23.2%), HAstV-3 (3.6%), HAstV-4 (14.3%) and HAstVs -5, -6, -7 and -8 (1.8% each) were detected. In Goiânia and Campo Grande, HAstV-1 was the most frequently detected genotype while in Brasília (DF) it was HAstV-2. Shifts in the circulation of astrovirus genotypes were observed in DF and Campo Grande. All samples collected by rectal swabs were viral negative. The astrovirus genotypes were detected in all age groups and there was no correlation between genotype and age group.

Keywords
astrovirus - genotyping - sequencing - nested-PCR - Brazil

 
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